PBK/SPK重组抗体[EP2520Y]_PBK/SPK抗体(ab75987)| Abcam中国

来自 : www.abcam.cn/pbkspk-antibody-e 发布时间：2021-03-25

All lanes : Anti-PBK/SPK antibody [EP2520Y] (ab75987) at 1/100000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PBK knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Lanes 1- 2: Merged signal (red and green). Green - ab75987 observed at 40 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab75987 was shown to react with PBK/SPK in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266827 (knockout cell lysate ab257575) was used. Wild-type HEK-293T and PBK knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab75987 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4 C at a 1 in 100000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H L (IRDye 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H L (IRDye 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Synthetic peptide within Human PBK/SPK (C terminal). The exact sequence is proprietary. 阳性对照 WB: HEK-293T, HeLa and HAP1 cell lysates. IHC-P: ...购买经敲除验证的重组PBK/SPK兔单克隆抗体[EP2520Y](ab75987)，PBK/SPK抗体经WB,IP验证，可与人样本反应。6篇文献引用，中国现货速达。PBK/SPK重组抗体[EP2520Y]